Coding
TSHantag

Part:BBa_K2404000:Design

Designed by: Ryan Coates   Group: iGEM17_Cardiff_Wales   (2017-09-12)


A Thyroid Stimulating Hormone antagonist without His-Tags


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794


Design Notes

We generated this part through the following steps:

- We ordered the TSH gBlock from IDT that included 5' and 3' BsmBI restriction sites

- This was introduced into BBa_P10500 by digestion with BsmBI. This generated a 5' and 3' BsaI sites for level 1 cloning


Source

Synthetic G-block. This modified protein was discovered in a paper by Fares et al in 2001.

References